Gene Expression |
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● RNA should be transferred in dry ice.
● Cultured cells, tissue, biopsy and PBMC should be sent in Liquid Nitrogen or RNA later with dry ice
● samples in which gene of interest is expressed ,concentration of cDNA has to be 2 ug/100 ul
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Xcelris Genomics provides a comprehensive range of gene expression analysis from bacteria, yeast, fungi, cell line and tissue of plant, animal, human built on multiple platforms including Genome Lab GeXP and QRT-PCR to address your scientific goals. |
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Procedure
Xcelris offers Gene Expression by the following methodology:
● Quality Check analysis of samples
● PCR Amplification using cDNA to check amplification
● Real time PCR using SYBR green assay
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| Data Analysis & Deliverables |
Xcelris will provide the customers with data analysis using Δct value to determine fold changes in gene expression against house keeping gene. |
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| Please contact: bdgenomics.corp@xcelrislabs.com |
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