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ION Torrent: Personal Genome Machine (PGM™) System

Personal Genome Machine
About Ion PGM Sequencer
The Personal Genome Machine (PGM™) combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data, democratizing sequencing and providing scalability and flexibility for various applications.
The PGM™ sequencer can use multiple ion sequencing chips to produce 10 Mb to 1000 Mb of sequence data depending upon the chip selected.
Ion PGM™ sequencer exhibits superior long-read accuracy yielding 99+% raw ac­curacy on single reads and over 99.97% consensus accuracy in about 2.5 to 4.5 hours, the fastest next generation sequencing run time available.
The data is analyzed on Torrent Server to convert raw signal into base calls typically in less than 1 hour. The Torrent Suite Software performs preliminary reference genome alignment and outputs data in FASTQ and SFF formats for easy downstream analysis with third party analysis tools.
Data Out Put
Chip Type
Data Generation in MB
Read Length in Base
upto 100
upto 200
upto 500
upto 1000
Applications at XGC
Whole genome de novo and re-sequencing for viral, bacterial and fungal genomes.
Transcriptome analysis for bacteria and fungus.
Microsatellite discovery by genome shotgun sequencing.
Targeted sequencing for SNP detection using Agilent SureSelect Target Capture method.



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