ION Torrent: Personal Genome Machine (PGM™) System |
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Personal Genome Machine
(PGM™) |
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About Ion PGM Sequencer |
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The Personal Genome Machine (PGM™) combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data, democratizing sequencing and providing scalability and flexibility for various applications. |
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The PGM™ sequencer can use multiple ion sequencing chips to produce 10 Mb to 1000 Mb of sequence data depending upon the chip selected. |
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Ion PGM™ sequencer exhibits superior long-read accuracy yielding 99+% raw accuracy on single reads and over 99.97% consensus accuracy in about 2.5 to 4.5 hours, the fastest next generation sequencing run time available. |
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The data is analyzed on Torrent Server to convert raw signal into base calls typically in less than 1 hour. The Torrent Suite Software performs preliminary reference genome alignment and outputs data in FASTQ and SFF formats for easy downstream analysis with third party analysis tools. |
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Data Out Put |
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Chip Type |
Data Generation in MB |
Read Length in Base |
316 |
upto 100 |
100 |
316 |
upto 200 |
200 |
318 |
upto 500 |
100 |
318 |
upto 1000 |
200 |
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Applications at XGC |
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Whole genome de novo and re-sequencing for viral, bacterial and fungal genomes. |
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Transcriptome analysis for bacteria and fungus. |
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Microsatellite discovery by genome shotgun sequencing. |
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Targeted sequencing for SNP detection using Agilent SureSelect Target Capture method. |
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