Medical Genetics Services
 
 
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XcelGen Endofree Plasmid Isolation Mini Kit
 
Cat No.: XG1212-01 • Preps: 50

Highlights
• Reduce endotoxin level up to 0.1EU/µg of plasmid DNA
• Purified DNA suitable for downstream applications

Description
XcelGen Endofree plasmid DNA isolation kit uses a specially formulated buffer for extraction of endotoxin from plasmid DNA. Special lysis buffer effectively removes impurities and other PCR inhibitors, making the purified DNA compatible for downstream applications.

Technical Specifications
Yield: Up to 12 µg of plasmid DNA
Preparation time: less than 60 minutes
Sample: 1-4 ml of recombinant E. Coli culture
Elution volume: 50-100 µl

Applications
PCR, Restriction Digestion, Hybridization, NGS Sample preparation, Sequencing
   
   
 
Manual           inquiry

XcelGen Plasmid Isolation Kit
 
Cat No.: XG1211-01 • Preps: 50

Highlights
• No phenol-cholroform extraction or alcohol precipitation
• Column based purification, no organic extraction
• Works on both High copy number and Low copy number plasmid
• Purified DNA suitable for Cloning, Sub-cloning, Sequencing, Transfection
   of robust cells such as HEK293 cells

Description
XcelGen plasmid isolation kit is designed for fast and efficient isolation of plasmid DNA from cultured bacterial cells. Special lysis buffer effectively removes impurities and other PCR inhibitors, making the purified DNA compatible for downstream applications.

Technical Specifications
Yield: Up to 12 µg of plasmid DNA
Preparation time: less than 30 minutes
Sample: 1-4 ml of recombinant E. Coli culture
Elution volume: 75-150 µl

Applications
PCR, Restriction Digestion, Hybridization, Sequencing
   
   
 
Manual           inquiry

XcelGen 96-well Plasmid Isolation Kit
 
Cat No.: XG1201-96 • Preps: 50

Highlights
• Fast: Process up to 96 samples in less than 90 minutes
• No phenol-cholroform extraction or alcohol precipitation
• Column based purification, no organic extraction
• Purified DNA suitable for Cloning, Sub-cloning, Sequencing, Transfection
   of robust cells such as HEK293 cells

Description
XcelGen 96-well plasmid isolation kit is designed for high-throughput plasmid DNA purification from 1-1.5 ml of cultured bacterial cells using an efficient 96-well Miniprep system. A modified alkaline lysis and RNase treatment are used to obtain clear cell lysate with minimal gnomic DNA/RNA contamination. Contaminants are removed with a wash buffer and the purified plasmid DNA is eluted by a low salt elution buffer.

Technical Specifications
Yield: Up to 8 µg of plasmid DNA
Preparation time: less than 60 minutes
Sample: 1-1.5 ml of E. Coli culture
Elution volume: 75-150 µl

Applications
PCR, Restriction Digestion, Hybridization, Sequencing
   
   
 
Manual           inquiry

XcelGen DNA Gel/ PCR Purification Kit
 
Cat No.: XG3511-01/XG3514 • Preps: 50

Highlights
• Broad fragment size: Suitable for fragment range of 100bp-20kb
• Fast: Simple and fast: DNA recovery is performed in less than 20
   minutes
• Efficient :Recovery up to 80-90%

Description
XcelGen DNA gel/PCR purification kit is designed for fast and efficient recovery of DNA from agarose gels, with removal PCR primers, primer-dimers, enzymes and PCR-buffers from PCR products.

Technical Specifications
Recovery: 80-90%
Preparation time: less than 60 minutes
Sample: DNA/PCR product
Elution volume: 30-50 µl

Applications
Sequencing, Ligation, Transformation, Restriction digestion, labelling, microinjection, PCR and in vitro transcription
   
   
 
Manual           inquiry

 
 
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