 Custom Oligo Synthesis Services |
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Xcelris Genomics as an asian market leader in delivering the products & services in Genomics, we are pleased to introduce to you our new service of customized DNA/RNA synthesis with New generation technology to improve the Quality & quantification of the current reasearch demand. |
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| Specifications of scale and yield of synthesis |
We offer different types of scales for the custom DNA synthesis |
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| Minimum Guarantee Yield in OD |
| Scale n mole |
Length |
Desalted |
RP1 |
HPLC |
PAGE |
| 10 |
15-34 |
2 |
N/A |
N/A |
N/A |
| 25 |
15-34 |
3 |
N/A |
N/A |
N/A |
| 50 |
5-00 |
5 |
2 |
2 |
0.5 |
| 100 |
5-00 |
10 |
4 |
4 |
1.0 |
| 200 |
5-120 |
14 |
6 |
6 |
2.0 |
| 1000 |
5-120 |
40 |
13 |
13 |
5.0 |
|
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| Purification |
We offer different types of purification for the customized applications,as well as standard RAPD Kits for the researcher's demand.
● Desalt,
● HPLC
● PAGE
|
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| Desalt |
Catridge purification is extremely precise technology method based on the reverse phase chromotography. Oligos are quantitated by UV spectrophotometry/Nano-Drop Technology to provide an accurate measure of yield in Optical density (OD). This varies with length and decreases for shorter oligos. All oligos are quality controlled (QC) and checked by Mass spectrometry. |
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| HPLC |
Xcelris offers HPLC Purification for Modified & Unmodified oligos for an advanced technology with RP-HPLC in high resolution & high intensity due to chromatographic separation for the cloning application. With this technology we can separate n-x & most truncated sequence created during synthesis, thus increasing better productivity. |
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| PAGE |
Xcelris offers unique PAGE purification for longer oligonucleotides ,which requires highest level of purification for the most sensible reactions. In PAGE technique, it removes most truncated failures during synthesis, and achieves 95% purity, better than any other purification method. |
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| Modification Services |
Xcelris offers Modifications for the Customer's requirement with different applications.
● Non-fluoroscent modifications
● Back bone-sugar-Base modifications.
● Fluoroscent Dye modifications
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| Non-fluoroscent modifications |
| Modification |
5' |
3' |
Int |
| AminolinkC6 |
x |
|
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| AminolinkC12 |
x |
|
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| AminolinkC3/C6/C7 |
|
x |
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| Biotin3' |
|
x |
|
| Biotin5' |
x |
|
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| Biotin TEG |
x |
x |
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| Digoxigenin |
x |
x |
x |
| Phosphorylation(PHOSs) |
x |
x |
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| Thiol Modifier |
x |
x |
|
|
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| Back bone-sugar-Base modifications |
| Modification |
5' |
3' |
Int |
| Phosphorothiote Bses(PTO) |
x |
x |
x |
| 2'-Deoxyinosine (INO) |
|
x |
x |
| 2'-Deoxyinosine (URI) |
|
x |
x |
| 5-Methyl-dc |
|
x |
x |
| AminoC6-dT |
|
|
x |
| Biotin-dT |
|
|
x |
| dSpacer (Deoxyabasic) |
|
|
x |
| 5'ddA:5'ddG:5'ddC5'ddt |
|
x |
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| 2'3'dideoxyC(3'ddc) |
|
x |
|
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| Fluorescent dyes at a glance |
Modification |
| Dye |
Abs |
Em |
Ext.Coeff. |
Mol.Weight |
5' |
Int |
3' |
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|
|
|
|
|
|
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| Fam |
495 |
520 |
83 |
474,50 |
x |
|
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| FITC |
495 |
520 |
79 |
505,00 |
x |
x |
x |
| Fluorescein |
495 |
520 |
83 |
504,00 |
|
|
x |
| Fluorescein-dT |
494 |
522 |
75 |
472,71 |
|
x |
|
| TET |
521 |
536 |
73 |
612,30 |
x |
|
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| JOE |
520 |
548 |
73 |
603,40 |
x |
x |
x |
| Cy3 |
552 |
570 |
150 |
444,60 |
x |
|
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| TAMRA |
544 |
576 |
90 |
512,58/612,70 |
X |
|
X |
| Rhodamine Red |
560 |
580 |
129 |
770,00 |
x |
x |
x |
| ROX |
575 |
602 |
82 |
632,78 |
x |
x |
x |
| Texas Red |
583 |
603 |
116 |
818,00 |
x |
x |
x |
| Cy3.5 |
588 |
604 |
150 |
544,70 |
x |
|
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| Cy5 |
649 |
670 |
250 |
470,63 |
x |
|
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| Quencher |
|
Quencher Range |
Molar Ext.Coeff |
Mol.Weight |
|
|
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| BHQ1 |
534 |
480-580 |
34.00 M-1 cm-1 |
491.5 g/mol |
|
|
x |
| BHQ2 |
544 |
520-600 |
91.000 M-1 cm-1 |
493.5 g/mol |
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|
x |
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| Delivery Options |
Our Customised Oligos after purification process in mods / unmods are shipped Lyophilised in the tube . In plate format, the oligos are Dissloved / Lyophilised as per the customer's requirement along with Synthesis Report & Quality Document. |
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Once you fill up the enquiry form we will provide you with the quotation. |
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