R-E. coli Sequencing / BAC Sequencing |
|
● Details of Plasmid Insert size & ‰ GC content
● Reverse and Forward Primer
● Vector details & Antibiotic details
|
|
|
| |
Xcelris offers R-E coli sequencing by the following methodology |
| |
Procedure
● Inoculation in Media
● Isolation of Plasmid
● QC on agarose Gel
● Primer design and synthesis
● DNA Sequencing by using 96 capillary high throughput sequencer; ABI 3730 XL to generate sequences
with accurate base calling
● Date presentation in .abi or PDF format
Delivery of results
DNA Sequencing results will be delivered to the client through email (SCF or ABI Format). If client requires results in hard copies, it has to be mentioned on prior basis.
|
| |
|
|
| |
|
| |
