Medical Genetics Services
 
 
 
Plasmid/PCR Sequencing
Cat No.: SEQ-01

Process
•   Quality check of Plasmid/PCR DNA by gel electrophoresis for purity & integrity.
•   Sequencing of DNA with client specific primer or universal primer using Sanger sequencing
     chemistry.

Sample Requirement
Purified PCR product- 20 μl of 10-20 ng/μl per sample, plasmid- 25μl of 30-100ng/μl per sample with customer specified primers, conc.= 10 pmole/μl( μM), volume- 10 μl and Tm of the primers.

Turn Around Time
2-3 working days from the receipt of sample to Xcelris premises.

Deliverables
Q20 read length up to 800 bases with ABI or SCF, FASTA file.
 
 
 
Plasmid/PCR Sequencing Services   |   r-E.coli Culture Sequencing Services   |   Primer Walk Sequencing
Microsatellite Genotyping   |   SNP Genotyping by Sequencing on ABI3730xl   |   SNP Genotyping by SNaPshot Assay
Microbial identification services   |   Customized Services
 
 
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