Cat No.: SEQ-04
Primer walking is a step-by-step sequencing method of choice for sequencing DNA fragments between 2 and 4 kilobases. Such fragments are too long to be sequenced in a single sequence read using Sanger Sequencing method.

Process:
•   Primer design and synthesis.
•   BAC/Fosmid DNA isolation and sequencing with interim primers.
•   Universal primer details used for sequencing.
•   Q20 read length up to 800 bases with ABI or SCF, FASTA file.
•   Quality control analysis reports.

Sample Requirement:
4 to 8 μg of purified PCR product (1-4 kb) / plasmid (10 kb) with insert size up to 3-4 kb/glycerol stock of culture, details of inserted gene size and gene information, vector details, customer specified primers, conc.= 10 pmole/μl ( μM), volume- 20μl and Tm of the primers.

Turn Around Time:
10-15 days from the receipt of sample to Xcelris premises. Turn around time for primer walk will depend on number and size of the insert DNA.

Deliverables:
Q20 read length up to 800 bases with ABI or SCF, FASTA file.