Plant tissue sample

●  Total 3-4g tissue sample is required. ●  Make sure that before harvesting the sample all your vials for sample collection are properly labeled. ●  Plant tissue samples should be harvested and immediately washed with DEPC treated water. ●  After the sample is harvested and washed, it should be immediately immersed in RNAlater solution and     placed in dry ice. ●  The sample should be aliquoted in at least 15-20 vials of 2ml and placed at -80ºC.

Human/ Animal tissue sample

●  Total 2 gm tissue sample is required. ●  If sample is human biopsy tissue, the minimum requirement will depend upon the availability of tissue. ●  Make sure that the sample collection vials for sample collection are properly labeled. ●  Animal/Human tissue samples should be immediately collected and if required washed with DEPC treated     water. ●  Immerse the tissue samples in RNAlater solution and immediately place in dry ice at the collection site. ●  The sample should be aliquoted in 10 -15 vials of 2 ml and placed in -80ºC.

Blood Samples

If the sample comes in the form of body fluid e.g. blood. ●  It should be freshly collected (about 40 ml) in vacutainor with EDTA as anticoagulant. ●  It should be aliquoted atleast in 4 vacutainor of 9 ml each.     ●  For WTA analysis- 2 x 9 ml of blood sample is required.     ●  For small RNA analysis- 2 x 9 ml of blood sample is required. ●  Blood collected in EDTA vacutainor should be immediately place at 4°C.

Total RNA sample

●  Total RNA is isolated in a RNase free environment using Standard kits available in the market. ●  It should be of high quality (300-400µg) with concentration not less than 400ng/µl. ●  It should be aliquoted in at least 8 - 10 vials with minimum amount of 40µg of total RNA in each tube     having RIN value > 6.5.

Bacterial Culture

●  In case of bacteria, you can send us bacterial culture in 10-15% glycerol stock and should be immediately     transported to Xcelris genomics in dry ice. ●  Freshly isolated RNA (200-300 µg) is accepted only in case of pathogenic bacteria with RIN >7.0. Reference     literature for bacteria along with non-standard media composition details.

Storage Conditions during transportation

●  Freshly harvested tissue sample immersed in RNAlater solution should be immediately transported to     Xcelris Genomics in dry ice. ●  Total RNA samples should be immediately transported to Xcelris Genomics in dry ice along with gel picture     (1% denaturing agarose gel) and bioanalyzer profile on RNA 6000 nano chip to check quality of RNA. ●  Freshly collected blood in EDTA vacutainor should be immediately transported to Xcelris Genomics with cool     pack at 4°C condition.

Transcriptomics, or genome-wide expression profiling, aims to catalogue the complete set of RNA transcripts produced by the genome, including mRNAs, non-coding RNAs and small RNAs. Xcelris Genomics provides you with one stop solution for your transcriptome sequencing by using Next Generation technologies like ABI SOLiD, Roche GS FLX system Titanium and Illumina High Seq 2000 platforms. We provide RNA sequencing which allows the profiling of the whole population of mRNA in any eukaryotic species and enables mapping and digital quantification of whole transcripts. It also provides a rich source of sequence data for assessing alternative splice events High throughput sequence barcoding methodology of transcriptome assay enables genome wide expression profiling with high sensitivity and a wider dynamic range than microarray technology. The next generation sequencing based whole transcriptome analysis enables to detect expression of all coding, n on-coding and novel RNA's, Identification of alternative splicing events and determination of identifying allelic specific expression patterns.

Along with the above the customers receive a quality report which includes all the details of the samples that has been received. Xcelris Genomics also offers an advanced Bioinformatics Analysis for reference based whole trascriptome analysis and de novo whole transcriptome analysis based on the customer requirement.