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Microbial Identification Service

Cat No.: MID-S/C/R

Microbial Identification Service is based on sequencing of the 16SrRNA gene (bacterial) and ITS/D2 region of 28S Large subunit (LSU) rRNA gene for (Fungal/Yeast/Algae) phylogenetic analysis.

Types of Microbial Identification Service:
MID-S: This service includes sequencing of ribosomal genes or other conserved region of up to 1500 bp and its sequencing data with chromatogram and FASTA file.

MID-C: This service includes sequencing of ribosomal genes or other conserved region of up to 1500 bp and its contig report with sequencing data consisting of chromatogram and FASTA file.

MID-R: This service includes sequencing of ribosomal genes or other conserved region of up to 1500 bp and its comprehensive report includes genus and species level identification with phylogenetic tree and distance matrix generated using MEGA 4.1 for representation of the relationships, to obtain organism lineage and taxonomy.

Process:

•	Genomic DNA extraction from microbial culture & amplification of the desired gene by PCR.
•	Enzymatic/Gel based purification of the amplicon.
•	Sequence data generation by primer walk using BDTv3.1 chemistry on ABI 3730xl.
•	Quality check, trimming of low quality sequence from ends and assembly.

Sample Requirement

(a)	Pure culture propagation: for Bacteria/Yeast/Fungi (Plate/Slants/Glycerol stock), culture growth conditions (medium / temperature)
(b)	Isolated microbial genomic DNA: Concentration of 100 - 200 ng/μl , volume:- 20 μl
(c)	PCR product of 16S/18S rDNA gene: concentration of 20-30 ng/μl, volume:- 20 μl.

Turn Around Time:
3-12 working days from the receipt of sample to Xcelris premises.

Deliverables:
The Report will include: Genus and species level identification with top ten matches and DNA consensus sequence along with electropherogram, phylogenetic tree and distance matrix generated using MEGA 4.1 for representation of the relationships, to obtain organism lineage and taxonomy.

Sample Submission Form

Sample Submission Form

Plasmid/PCR Sequencing Services | r-E.coli Culture Sequencing Services | Primer Walk Sequencing

Microsatellite Genotyping | SNP Genotyping by Sequencing on ABI3730xl | SNP Genotyping by SNaPshot Assay

Microbial identification services | Customized Services